Xenonuclear Nuclear Transfer Embryos reconstructed by Bovine Oocytes and Camel (Camelus Bactrianus) Skin Fibroblasts and Their Subsequent Development
نویسندگان
چکیده
منابع مشابه
Monophyletic origin of domestic bactrian camel (Camelus bactrianus) and its evolutionary relationship with the extant wild camel (Camelus bactrianus ferus)
The evolutionary relationship between the domestic bactrian camel and the extant wild two-humped camel and the factual origin of the domestic bactrian camel remain elusive. We determined the sequence of mitochondrial cytb gene from 21 camel samples, including 18 domestic camels (three Camelus bactrianus xinjiang, three Camelus bactrianus sunite, three Camelus bactrianus alashan, three Camelus b...
متن کاملEffect of activation treatments of recipient oocytes on subsequent development of bovine nuclear transfer embryos.
Effects of recipient oocyte activation methods on the development of nuclear transfer (NT) embryos were investigated. In Exp. 1, cell-cycle phase of serum-starved bovine cumulus cells was examined by flow cytometry. Majority (95.5%) of medium-sized (16-20 microm) cells that made up 56% of total cells was at the G0/G1 phase. NT embryos were constructed by electric fusion with the medium-sized se...
متن کاملIdentification of SNPs and their validation in camel (Camelus bactrianus and Camelus dromedarius)
The dromedary (Camelus dromedarius) and the bactrian camel (Camelus bactrianus) are among the last species that have been domesticated around 3000–6000 years ago. To understand relationship between genetic and phenotypic variations in camel, single nucleotide polymorphism (SNP) markers covering the coding part of genome were developed. These gene-associated SNPs can themselves be causative SNPs...
متن کاملDevelopment of bovine embryos reconstructed by nuclear transfer of transfected and non-transfected adult fibroblast cells.
An association of two techniques, nuclear transfer (NT), and transfection of somatic animal cells, has numerous potential applications and considerable impact, mainly in agriculture, medicine, pharmacy, and fundamental biology. In addition, somatic cell nuclear transfer is the most efficient alternative to produce large transgenic animals. We compared in vitro and in vivo developmental capaciti...
متن کاملIn vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studies of iSCNT using murine cells and bovine oocytes have been reported, the development of murine-bovine iSCNT embryos beyond the 8-cell stage has not been successful. In this paper, we examined the d...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: In Vitro Cellular and Developmental Biology--Animal
سال: 2005
ISSN: 1071-2690,1543-706X
DOI: 10.1290/0506038